The Central Analytical Platform for Mass Spectrometry of the Department of Chemistry provides a variety of instruments for the gas phase characterization of precursor molecules which is a standart analytical technique used by the working group Mathur.

Please check the platforms' webpage for more information about the available instruments and measurement techniques.

Platform for Mass Spectrometry

HPLC is an analytical technique which is used to separate, identify, and quantify each organic component in a mixture. A pressurized mobil phase,  eg. a mixture of H₂O/Me-OH in a specific ratio, contains  the sample mixture and is pumped through a column which is filled with an absorbing material. Actually an unpolar RP-18 reversed phase is installed. That leads to short retarding times of polar substances.

The components  of the analysis mixture are interacting slightly differently with the solid adsorbent material in the column (Stationary Phase). The different flow rate of the different components leads to the separation of the components as they flow out the column.

The detection happens by using an UV-VIS detector at the end of the system after the substances passed the column.

Available Knauer HPLC System:

•  Up to 4 solvents as a mobile phase programmable as a gradient. Actually H₂O and Me-OH is installed.
• Gradient Manager module provides a degasser to remove bubbles an set the gradient of liquid phase solvents.
• HPLC Pump with an analytical 10 ml pumphead
• Autosampler for 96 vials with flushing-  and dilution function
• Programmable column ofen

·         UV-VIS detector with wavelength between 190 nm and 780 nm. The detector has a “stopflow function” and is able to scan uv-vis spectra of a separated substance which is stopped in the measuring cell for identification works.